AO/EB Double Staining Kit Size:100T application dilution species ICC 1:500

application dilution species ICC 1:500 WB 1:5000 ICFCM 1:500 IHC-P 1:500 IP 1:50

the lysate was added at a ratio of 100 microliters of lysate per 2 million cells

with C-terminal mouse IgG2a Fc LEVPNGPWRSLTFYPAWLTVSEGANATFTCSLSNWSEDLMLNWNRLSPSNQTEKQAAFCNGLSQPVQDARFQIIQLPNRHDFHMNILDTRRNDSGIYLCGAISLHPKAKIEESPGAELVVTERILETSTRYPSPSPKPEGRFQIEGRMDPEPRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVTCVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEFKCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIYVEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHTTKSFSRTPGK

Dye Name wavelength Similar fluorescent dyes Recommended Color Settings Excitation wavelength Emission wavelength DAPI 360 461 DAPI                  TSA Monochromatic fluorescent dye 480 450 480 Opal480/Aqua                  TSA Monochromatic fluorescent dye 520 490 520 FITC/AF488/Opal520                  TSA Monochromatic fluorescent dye 540 520 540 AF514/Opal540                  TSA Monochromatic fluorescent dye 570 550 570 CY3/AF555/Opal570                  TSA Monochromatic fluorescent dye 620 590 620 AF594/Opal620/TexasRed                  TSA Monochromatic fluorescent dye 650 630 650 CY5/AF610/Opal650                  TSA Monochromatic fluorescent dye 690 640 670 AF647/CY5/Opal670                  TSA Monochromatic fluorescent dye 700 680 702 AF680/CY5

AO/EB Double Staining Kit Size:100T application dilution species ICC 1:500Product Specification Usage Self prepared material: Fluorescence microscope; PBS; Cell counting board; Glass slide; Cover glass slides 1. Preparation of staining working solution Before the formal experiment, take acridine orange staining solution (reagent A), ethidium bromide staining solution (reagent B), and dilution buffer solution (reagent C), and dilute them to the required AO EB staining working solution in the ratio of A: B: C=1: 1: 8 2. Cell