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Background Cytochrome P450 oxidoreductase (CPR)
on endothelium
Careful sample handling (centrifugation
(6) Incubate the plate at 37°C for up to 30 min
UniOne® TR-FRET Human TNF-α/TNFR2 Binding Kit WB Background Cytochrome P450 oxidoreductase (CPR)Product Specification Host Human Stability & Storage 80 Background This kit employsHomogeneous Time Resolved Fluorescence (TR FRET) technology to measure the interactionbetween TNF and TNFR2. The method enables simple and rapid high throughputscreening of inhibitors and antibody blockers. As shown in thefigure, the TNF TNFR2 interaction is detected using an Eu labeled anti Tag1antibody (TR FRET donor) and an Acceptor labeled TNF protein (TR
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