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insufficient Wash as per instructions 2 Substrate 3
Centrifuge washing with pre-cooled PBS and collect 1-10 × 105Cells include cells in the culture supernatant
horseradish peroxidase-labeled streptavidin was added (Streptavidin-HRP ， SA-HRP) 。 After washing
so wash board 5 times
Human pro-GRP, His Tag Size:10μg insufficient Wash as per instructionsProduct Specification Species Human Accession P07492 Amino Acid Sequence Protein sequence(P07492, Ser54 Asp121 with C 10*His) STGESSSVSERGSLKQQLREYIRWEEAARNLLGLIEAKENRNHQPPQPKALGNQQPSWDSEDSSNFKDGGGGSHHHHHHHHHH Expression System HEK293 Molecular Weight Theoretical: 9. 4kDa Actual: 13kDa Purity 95% by SDS PAGE Endotoxin <1EU g Conjugation Unconjugated Tag His Tag Physical Appearance Lyophilized Powder Reconstitution Reconstitute no more than 0. 1 mg mL