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and HRP conjugate are sequentially added to microwells pre-coated with an Osteoprotegerin (OPG) capture antibody
with C-terminal 8*His DLAKPSKLVNCTCESPHCKRPFCQGSWCTVVLVREQGRHPQVYRGCGSLNQELCLGRPTEFLNHHCCYRSFCNHNVSLMLEATQTPSEEPEVDAHLPGGGSHHHHHHHH
TGF-beta-1
The A405 of the sample minus the A405 of the blank control is the absorbance produced by pNA catalyzed by caspase 4 in the sample
5-HT ELISA Kit Organoid Kit and HRP conjugate are sequentiallyProduct Specification Usage 1. Sample collection preparation and preservation Serum: Whole blood sample placed at room temperature 2 Hour or 4C Overnight after 1000g Centrifugation 20 Minutes, take the supernatant to detect. The blood collection tubes shall be disposable non pyrogenic, non endotoxin tubes. deposit 20C Or 80C Storage, avoid repeated freezing and thawing. Plasma: Sample after collection 30 Within minutes 2 8C 1000g Centrifugation 15