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In non-small cell lung cancer (NSCLC)
RANKL functions by binding to its receptor RANK on the surface of osteoclast precursors
The color development solution of the substrate should be colorless or very light in color
with its N-terminus extending into the extracellular space and the C-terminus residing intracellularly
StartScript® One Step RT-PCR Kit Other Cytokines In non-small cell lung cancerProduct Specification Stability & Storage Store at 25 ~ 15 for 2 years Reference Components Component UA070126 50 Rxns UA070126 250 Rxns One Step Enzyme Mix (25X) 100 l 5 100 l One Step Reaction Mix (2X) 2 625 l 10 625 l RNase Free dH2O 2 1 ml 10 1 ml Protocol 1. Prepare the following mixture in an RNase free centrifuge tube: Component Volume One Step Enzyme Mix (25X) 2 l One Step Reaction Mix (2X) 25 l Gene specific Forward Primer (10 M) 2 l Gene
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