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the Cell Tracker Green was diluted 1: 1000 to 1 × Assay Buffer and the dose was expanded accordingly for large sample size determination
especially in endothelial cells
In humans it consists of one pair (2Ig-B7-H3) or two identical pairs (4Ig-B7-H3) due to exon duplication
Each component of the kit was left to equilibrate at room temperature for 30 minutes before operation was started
Human IL-17A, His tag Secondary Antibody the Cell Tracker Green wasProduct Specification Species Human Synonyms Cytotoxic T lymphocyte associated antigen 8 (CTLA 8) Accession Q16552 Amino Acid Sequence Protein sequence(Q16552, Gly24 Ala155, with C 10*His) GITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVAGGGGSHHHHHHHHHH Expression System HEK293 Molecular Weight Theoretical: 16. 8kDa Actual: 17, 20kDa Purity 95% by SDS PAGE Endotoxin
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